Technology Platform

NIR-II Technology Principle

The differential absorption and scattering of light by biological tissue at different wavelengths determines the "readability" of a detection window. Conventional IVD relies on visible light (400–780 nm) and NIR-I (700–900 nm) bands, which suffer from significant biological autofluorescence (background noise) and tissue scattering (signal attenuation), limiting assay sensitivity and quantitative precision.

NIR-II fluorescence (1000–1700 nm) offers deeper tissue penetration, lower background noise, and higher signal-to-noise ratio compared to visible light and NIR-I. WWHS uses a proprietary encapsulation process to stably encapsulate hydrophobic NIR-II dyes inside polymer microspheres, producing monodisperse functionalized nanobeads with fluorescence quantum yield exceeding 20%. Infrared fluorescence POCT reagents based on these beads are excited at 780 nm and detected at wavelengths above 1000 nm. Compared to conventional methods, NIR-II technology delivers superior anti-interference performance, fully eliminating sample autofluorescence interference. Leveraging the strong penetration of infrared light, it enables 3D volumetric signal detection, reduces fluorescence scattering effects, and significantly boosts assay sensitivity.

• Physical PrincipleNIR-II fluorescence imaging: deep tissue penetration, low background noise
• Complex SamplesStable and precise quantification even with hemolytic, icteric, and lipemic samples
• Foundational InnovationDerived from the pioneering NIR-II fluorescence imaging principle developed by Prof. Hongjie Dai's team